Tunnel Hill State Trail stretches for 45 miles from Harrisburg to Karnak. The trail continues on a trails spur for 2.5 miles from Karnak to Cache River State Natural Area - Henry Barkhausen Wetlands Center on the old Chicago and Eastern Illinois railroad bed.

The 9.3-mile section between Tunnel Hill and Vienna crosses trails already known to outdoor recreationists: the River-to-River Trails, which extends from the Mississippi to the Ohio River; the unmarked American Discovery Trails, which in southern Illinois follows back roads and some of the River-to-River Trail; the U.S. 76 Bicycle Route, a part of the TransAmerica Bike Route; and the Trail of Tears, the primary route the Cherokee Indian tribe took in the winter of 1838-39 during their forced move from the Great Smokies to Oklahoma.